There were four biblical characters called Amasai (burdensome).

• Amasai the Levite, son of Elkanah, of the ancestry of Samuel (1 Chr. 6:25, 35).
• An Amasai who was the leader of a body of men who joined David in the "stronghold," probably of Adullam (1 Chr. 12:18).
• Amasai the priest who was appointed to precede the ark with blowing of trumpets on its removal from the house of Obed-edom (1 Chr. 15:24).
• Amasai, the father of a Levite, one of the two Kohathites who took a prominent part at the instance of Hezekiah in the cleansing of the temple (2 Chr. 29:12).