The Amboise conspiracy, or Tumult of Amboise (1560), was a failed attempt by Huguenots and the house of Bourbon to wrest power over France, by abducting the young king, Francis II and arresting François (the Duke of Guise) and his brother Charles (cardinal of Lorraine).

Rumors of the plot reached Charles ahead of time, and the conspirators were massacred. In the following weeks, 1200 bodies hung on iron hooks on the façade of the Château d'Amboise and from nearby trees, enraging the Huguenot opponents of the house of Guise and adding to the tensions which culminated in the French Wars of Religion.