The Fox-Wisconsin Waterway is a waterway formed by the Fox and Wisconsin Rivers. First used by white men in 1673 during the expedition of Marquette & Joliet, it was one of the principal routes used by travelers moving between the Great Lakes and the Mississippi River until the completion of the Illinois and Michigan Canal in 1848. The Fox-Wisconsin Waterway was traveled by taking a small boat or canoe into Green Bay and entering the Fox River, following it through Lake Winnebago and continuing on the Fox until reaching Portage, Wisconsin, where the travelers would carry their boats and and supplies a few miles west and resume boating on the Wisconsin River, continuing upon it until reaching the Mississippi at Prairie du Chien, Wisconsin.

The Fox-Wisconsin Waterway was replaced by canals and railroads in the 19th Century, and is no longer used as a transportation route between the Mississippi River and the Great Lakes.