Luigi Di Biagio (born June 3, 1971 in Rome) is an Italian football (soccer) defensive midfielder, who currently plays for Brescia of Serie A. Di Biagio is most recognized by missing a decisive kick in the penalty shootout for Italy against France in the 1998 World Cup.

Prior to joining Brescia, Di Biagio has played for Lazio (1988-89), Monza (1989-92), Foggia (1992-95), AS Roma (1995-99), and Internazionale (1999-2003). He has been capped 31 times for Italy, scoring two goals. Other than the 1998 World Cup, he also played for his country in the 2002 edition, as well as Euro 2000, when he did connect in a penalty shootout against the Netherlands: it was the first penalty he shot after the infamous miss during 1998 World Cup.